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anti lamin a c  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti lamin a c
    Anti Lamin A C, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamin+a+c/pmc13019076-412-10-12
    Average 86 stars, based on 1 article reviews
    anti lamin a c - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cells
    Article Snippet: .. After a brief rinse with PBST, samples were incubated with the following primary antibodies overnight at 4 °C: phospho-myosin light chain 2 (Thr18/Ser19) (Cell Signaling Technology, #95777; 1:400), Lamin A/C (Cell Signaling Technology, #4777; 1:400), and YAP (Cell Signaling Technology, #4912; 1:500). .. After washing twice with PBST and three times with PBS, cells were incubated with Alexa Fluor 568-conjugated anti-rabbit IgG (Thermo Fisher Scientific, A-11011, 1:400) or Alexa Fluor 647-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A-32728, 1:400) for 1 h at room temperature.

    Article Title: Induction of adipocyte thermogenic program by Rho-associated coiled-coil containing kinase 2
    Article Snippet: Equal amounts of protein (20 μg) per sample were subjected to SDS-PAGE (30% Acrylamide/Bis Solution, Bio-Rad; 1610158) and transferred to PVDF membranes (Millipore; IPVH00010). .. The PVDF membranes were blocked with 5% nonfat dry milk (Research Products International; M17200 ) in TBST for 1 hour and then incubated overnight at 4°C with primary antibodies against ROCK1 (BD Biosciences; 611136), ROCK2 (BD Biosciences; 610623), phospho-Thr 850 MBS (Millipore; 36-003), total MBS (BioLegend; 925101), UCP1 (Abcam; ab23841), phospho-FOXO1 (Ser 256 , Cell Signaling; 9461), FOXO1 (Cell Signaling; 2880), phospho-SAPK/JNK (Thr 183 /Tyr 185 , Cell Signaling; 9251), phospho-AKT (Ser473, Cell Signaling Technology; 4060), AKT (Cell Signaling; 9272), total JNK (SAPK/JNK, Cell Signaling; 9252), Lamin A/C (Cell Signaling; 4777), and GAPDH (Cell Signaling; 2118). ..

    Article Title: Induction of Adipocyte Thermogenic Program by Rho-associated Coiled-coil Containing Kinase 2
    Article Snippet: Equal amounts of protein (20 g) per sample were subjected to SDS-PAGE (30% Acrylamide/Bis Solution, Bio-Rad; 1610158) and transferred to PVDF membranes (Millipore; IPVH00010). .. The PVDF membranes were blocked with 5% nonfat dry milk (Research Products International; M17200) in TBST for 1 hour and then incubated overnight at 4°C with primary antibodies against ROCK1 (BD Biosciences; 611136), ROCK2 (BD Biosciences; 610623), phospho-Thr850 MBS (Millipore; 36-003), total MBS (BioLegend; 925101), UCP1 (Abcam; ab23841), phospho- Jo urn al Pr e-p roo f FOXO1 (Ser256, Cell Signaling; 9461), FOXO1 (Cell Signaling; 2880), phospho-SAPK/JNK (Thr183/Tyr185, Cell Signaling; 9251), phospho-AKT (Ser473, Cell Signaling Technology; 4060), AKT (Cell Signaling; 9272), total JNK (SAPK/JNK, Cell Signaling; 9252), Lamin A/C (Cell Signaling; 4777), and GAPDH (Cell Signaling; 2118). ..

    Article Title: Pck1 Deficiency Drives Mitochondrial Dysfunction and Cellular Senescence in Adipocytes.
    Article Snippet: Primary antibodies used were Pck1 (1:1000, CST, 12940), p21 (1:500, Santa cruz, sc- 6246), p16 (1:500, Santa cruz, sc- 1661), p- AKT (T308) (1:1000, CST, 2695), AKT (1:1000, CST, 4685), β- Actin (1:2000, CST, 4970), cGAS (1:1000, CST, D1D3G), p- STING (1:1000, CST, 72971), STING (1:1500, CST, 13647), pIRF3 (1:1000, CST, 29047), IRF3 (1:1000, CST, 4302), p- TBK1 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.70462 by IN A SP - N E PA L , W iley O nline L ibrary on [01/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 16 of 19 Aging Cell, 2026 (1:1000, CST, 5483), TBK1 (1:1000, CST, 3504), Tomm20 (1:2000, CST, 42406), Lamin A/C (1:2000, CST, 2032), GAPDH (1:2000, CST, 97166), Total mitochondrial protein cocktail (1:1000, Abcam, ab110413), and incubated at 4°C overnight. .. The secondary antibodies used were incubated at room temperature for 1 h, blots were imaged by Amersham ImageQuant 800 Protein Blotting Imaging System.

    Article Title: Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cells
    Article Snippet: .. Non-specific binding was blocked by incubation of the samples with 1 % (w/v) bovine serum albumin (BSA) in PBST overnight at 4 ◦ C. After a brief rinse with PBST, samples were incubated with the following primary antibodies overnight at 4 ◦ C: phospho-myosin light chain 2 (Thr18/Ser19) (Cell Signaling Technology, #95777; 1:400), Lamin A/C (Cell Signaling Technology, #4777; 1:400), and YAP (Cell Signaling Technology, #4912; 1:500). .. After washing twice with PBST and three times with PBS, cells were incubated with Alexa Fluor 568- conjugated anti-rabbit IgG (Thermo Fisher Scientific, A-11011, 1:400) or Alexa Fluor 647-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A-32728, 1:400) for 1 h at room temperature.

    Article Title: Activity-based CO 2 sensing using CarboSenR2 provides new insights into cellular metabolism
    Article Snippet: Membranes were washed in Revert 700 wash solution and imaged at 700 nm (LI-COR), then destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting. .. Membranes were blocked in 5% milk in TBST for 1 h followed by overnight incubation in primary antibody (1:1000) at 4 °C (phospho Acetyl-CoA Carboxylase (Ser79) (CST-3661S, Cell Signalling), PGC-1a (A12348, ABclonal), Lamin A/C (CST-4777S, Cell Signalling), mitochondrial cytochrome c oxidase subunit 1 (ab14705, Abcam)). .. Fluorescent secondary goat anti-mouse or anti-rabbit antibody (IgG (H + L) Goat anti-Rabbit, DyLight 800 4X PEG,: 10733944, Fisher Scientific, IgG (H + L) Cross-Adsorbed Goat anti-Mouse, DyLight 680,: 10797775, Fisher Scientific) incubations were performed at a 1:2000 dilution and imaged at 680 nm or 800 nm.

    Western Blot:

    Article Title: Oncogenes and tumor suppressor genes are enriched in stop-loss mutations generating protein extensions
    Article Snippet: .. Fraction purity was validated by immunoblotting using Hsp90 (Cell Signaling Technology, #4874, 1:1000) as cytoplasmic markers, and lamin A/C (Cell Signaling Technology, #2032, 1:1000) and c-myc (Cell Signaling Technology, #5605, 1:1000) as nuclear markers. ..

    Binding Assay:

    Article Title: Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cells
    Article Snippet: .. Non-specific binding was blocked by incubation of the samples with 1 % (w/v) bovine serum albumin (BSA) in PBST overnight at 4 ◦ C. After a brief rinse with PBST, samples were incubated with the following primary antibodies overnight at 4 ◦ C: phospho-myosin light chain 2 (Thr18/Ser19) (Cell Signaling Technology, #95777; 1:400), Lamin A/C (Cell Signaling Technology, #4777; 1:400), and YAP (Cell Signaling Technology, #4912; 1:500). .. After washing twice with PBST and three times with PBS, cells were incubated with Alexa Fluor 568- conjugated anti-rabbit IgG (Thermo Fisher Scientific, A-11011, 1:400) or Alexa Fluor 647-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A-32728, 1:400) for 1 h at room temperature.

    Immunofluorescence:

    Article Title: Lamin A/C safeguards replication initiation by orchestrating chromatin accessibility and PCNA recruitment.
    Article Snippet: .. For immunofluorescence, Primary antibodies against BrdU (BD, B44), PCNA (CST, 13110), Lamin A/C (CST, 4777), pH2AX (CST, 9718), pRPA-S33 (ab211877), and CTCF (Abcam, ab128873) were diluted 1:200 in PBS with 5% BSA and 0.5% Triton X-100. ..

    Pyrolysis Gas Chromatography:

    Article Title: Activity-based CO 2 sensing using CarboSenR2 provides new insights into cellular metabolism
    Article Snippet: Membranes were washed in Revert 700 wash solution and imaged at 700 nm (LI-COR), then destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting. .. Membranes were blocked in 5% milk in TBST for 1 h followed by overnight incubation in primary antibody (1:1000) at 4 °C (phospho Acetyl-CoA Carboxylase (Ser79) (CST-3661S, Cell Signalling), PGC-1a (A12348, ABclonal), Lamin A/C (CST-4777S, Cell Signalling), mitochondrial cytochrome c oxidase subunit 1 (ab14705, Abcam)). .. Fluorescent secondary goat anti-mouse or anti-rabbit antibody (IgG (H + L) Goat anti-Rabbit, DyLight 800 4X PEG,: 10733944, Fisher Scientific, IgG (H + L) Cross-Adsorbed Goat anti-Mouse, DyLight 680,: 10797775, Fisher Scientific) incubations were performed at a 1:2000 dilution and imaged at 680 nm or 800 nm.



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